THE USE OF IONIZED WATER IN THE MANAGEMENT OF INFECTED WOUNDS IN HORSES.
IS IT EFFECTIVE?
Horse wounds can be easily infected with bacteria depending on the nature of its cause such as laceration, abrasion, or puncture as well as the type of its environment. Various treatments are available in managing open wounds, including the usage of topical antibiotics and antiseptics. However, antibiotic resistance has been a major concern against the growth of common bacteria from horse wounds.
The antibacterial effect of electrolyzed and ionized waters of acidic pH on surface microbial growth reported by previous studies suggests that these substances can be used as an antiseptic or disinfectant. Its antibacterial effect has also been reported on mostly raw food products in food industries as well as animal transport vehicles. There is insufficient research on the usage of ionized water in veterinary practice. It is hypothesized that ionized water, depending on the pH, may serve as wound surface lavage solution that can control bacterial infection associated with chronic non-healing wound in horses. Therefore, the aim of this study is to investigate the effect of ionized water of different pH at different times against the growth of bacterial isolate from horse wounds.
Ten swab samples from equine infected wounds were collected, and bacterial isolation and identification were performed. The antibacterial effect of ionized water of pH 2.5, 4.5, 7.0 and 11.5 was tested on Staphylococcus aureus, Staphylococcus pseudintermedius, Staphylococcus intermedius, Escherichia coli, Pantoea agglomerans and Klebsiella pneumonia. The time-kill profiles of the ionized waters were determined at time 0, 2, 4, 6 and 8 hours.
Ionized water of pH 2.5 and 4.5 showed antibacterial activity against S. aureus, S. pseudintrmedius and S. intermedius with significant (p>0.05) reduction in colony-forming unit/mL within 2 to 8 hours. The degree of bactericidal effect of the acidic ionized water differs between species with S. intermedius being more susceptible. However, there was no antibacterial effect at pH 2.5, 4.5, 7.0 and 11.5 on the Gram-negative bacteria tested.
In conclusion, ionized water of pH 2.5 and 4.5 is effective in minimizing the growth of Gram-positive bacteria, and thus could be of clinical importance as an antiseptic for surface wound lavage in horses.
Date of Input: 02/09/2021 | Updated: 02/09/2021 | mjamil